The effect of operational variables (HRT and AT) on reactor performance and microbial diversity in laboratory-scale aerobic/anoxic up flow bioreactor

Ali Almasi, Mohammad Reza Zolfaghari, Zahra Bahman, Ali Akbar Zinatizadeh

Abstract


Background: Domination of microbial population is an effective parameter of biological process in the bioreactors. The aim of this study was to identify a domain microbial habitat in a sequential aerobic/anoxic bioreactor for the treatment of industrial waste water.

Methods: This study is an experimental one in which the degradation of organic matters was evaluated using laboratory scale aerobic/anoxic up flow bioreactor (UFBR) with an alternatively aeration action in sequence. Bacteriological study was carried out, such as a cultural-based technique, differentiation tests and biomass assessment procedure, detecting domain bacteria in the aerobic/anoxic conditions.

Results: The aerobic/anoxic treatment process resulted in the 45-95% BOD and 75-95% COD removal. The identified bacteries were studied as biological mass. The formed mass were found over than 109CFU/g of the flock forming in sludge built up, the majority of enumerated bacteria were facultative and anaerobic bacteria such as coli forms, micrococcaceae, Staphylococcus and Streptococci and Clostridium perfringens. The small portion of the defined population, less than 10% was detected as aerobic genera such as nitrifyers.

Conclusion: Anoxic condition affects the aerobic bacteria population in such a way that after aeration switching off they don’t have bioreactor in their structure. In opposite facultative and anaerobic bacteria were able flourish in building existence biomass inside the bioreactor. The benefits of this integrated bioreactor are the optimum efficiency which is coincided with no problem of the sludge build up.


Keywords


بیوتکنولوژی محیط

References


Xing DF, Ren NQ, Gong ML, Li JZ, Li QB. Monitoring of microbial community structure and succession in the biohydrogen production reactor by denaturing gradient gel electrophoresis (DGGE). Science in China Series C–Life Science. 2005;48(2):155-62.

Ren NQ, Xing DF, Rittmann EB, Zhao LH, Xie TH, Zhao X. Microbial community structure of ethanol type fermentation in bio-hydrogen production. Environmental Microbiology. 2007;9(5):1112-25.

Kraigher B, Kosjek T, Heath E, Kompare B, Mandic-Mulec I. Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors. Water Res. 2008;42(17):4578-88.

Ren L, Wu Y, Ren N, Zhang K, Xing D. Microbial community structure in an integrated A/O reactor treating diluted livestock wastewater during start-up period. J Environ Sci (China). 2010;22(5):656-62.

Pholchan MK, Baptista Jde C, Davenport RJ, Curtis TP. Systematic study of the effect of operating variables on reactor performance and microbial diversity in laboratory-scale activated sludge reactors. Water Res. 2010;44(5):1341-52.

Kaewpipat K, Grady CP Jr. Microbial population dynamics in laboratory-scale activated sludge reactors. Water Sci Technol. 2002;46(1-2):19-27.

Wagner M, Loy A. Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol. 2002;13(3):218-27.

Briones A, Raskin L. Diversity and dynamics of microbial communities in engineered environments and their implications for process stability. Curr Opin Biotechnol. 2003;14(3):270-6.

Wittebolle L, Boon N, Vanparys B, Heylen K, De Vos P, Verstraete W. Failure of the ammonia oxidation process in two pharmaceutical wastewater treatment plants is linked to shifts in the bacterial communities. J Appl Microbiol. 2005;99(5):997-1006.

Wang X, Wen X, Criddle C, Wells G, Zhang J, Zhao Y. Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems. J Environ Sci (China). 2010;22(4):627-34.

Villaverde S. Recent developments on biological nutrient removal processes for wastewater treatment. Reviews in Environmental Science and Bio/Technology. 2004;3:171-83.

Pala AI, Sponza DT. Biological treatment of petrochemical waste waters by Pseudomonas spp. added AS culture. Environ Technol. 1996;17:673-86.

Chisti Y. Pneumatically agitated bioreactors in industrial and environmental bioprocessing: Hydrodynamics, hydraulics and transport phenomena. Appl Mech Rev. 1998;51:33-112.

Burgess JE, Quarmby J, Stephenson T. Role of micronutrients in activated sludge-based biotreatment of industrial effluents. Biotechnol Adv. 1999;17(1):49-70.

Jorge G. Ibanez, Margarita Hernandez-Esparza, Carmen Doria-Serrano, Arturo Fregoso-Infante, and, Mono Mohan Singh, Biological Treatment of Pollutants and Wastes, Environmental Chemistry Fundamentals Part; 2007; 3: 276-295.

Sotomayor OA, Park SW, Garcia C. Software sensor for on-line estimation of the microbial activity in activated sludge systems. ISA Trans. 2002;41(2):127-43.

Baek JG, Park J, Kim TS, Park HD. Analysis of the time dependency of ammonia-oxidizing bacterial community dynamics in an activated sludge bioreactor. J Biosci Bioeng. 2011;112(2):166-9.

Huang JS, Wu CS, Chen CM. Microbial activity in a combined UASB-activated sludge reactor system. Chemosphere. 2005;61(7):1032-41.

Xing XH, Jun BH, Yanagida Mari, Tanji Y, Unno H. Effect of C/N values on microbial simultaneous removal of carbonaceous and nitrogenous substances in wastewater by single continuous-flow fluidized-bed bioreactor containing porous carrier particles. Biochemical Engineering Journal. 2000;5(1): 29-37.

Comeau Y, Hall KJ, Hancock REW, Oldham WK. Biochemical model for enhanced biological phosphorus removal. Water Res. 1986;20:1511-21.

Mino T, Tsuzuki Y, Matsuo T. Effect of phosphorus accumulation on acetate metabolism in the biological phosphorus removal process. In: Ramadori R. Proceedings of IAWPRC international conference on biological phosphate removal from wastewater, Rome, Adv. Water Pollut. Cont., Pergamon Press. 1987; 27-38.

Liu Y, Zhang T, Fang HH. Microbial community analysis and performance of a phosphate-removing activated sludge. Bioresour Technol. 2005;96(11):1205-14.

APHA, AWWA, WEF. Standard Methods forthe Examination of Waters and Wastewaters. 21st ed. Washington, DC: American Public Health Association (APHA); 2005.

Liu YJ, Wang XC, Yuan HL. Characterization of microbial communities in a fl utilized-pellet-bed bioreactor for wastewater treatment. Journal of Desalination. 2009;249:445-52.

Chandra R, Bharagava RN, Kapley A, Purohit HJ. Bacterial diversity, organic pollutants and their metabolites in two aeration lagoons of common effluent treatment plant (CETP) during the degradation and detoxification of tannery wastewater. Bioresour Technol. 2011;102(3):2333-41.

Keskes S, Hmaied F, Gannoun H, Bouallagui H, Godon JJ, Hamdi M. Performance of a submerged membrane bioreactor for the aerobic treatment of abattoir wastewater. Bioresour Technol. 2012;103(1):28-34.

Dytczak MA, Londry K L, Oleszkiewicz JA. Activated sludge operational regime has significant impact on the type of nitrifying community and its nitrification rates. Water Research. 2008;42(8-9):2320-8.

Fu Z, Yang F, An Y, Xue Y. Simultaneous nitrification and denitrification coupled with phosphorus removal in an modified anoxic/oxic-membrane bioreactor (A/O-MBR). Biochemical Engineering Journal. 2009; 43(2):191-6.




DOI: http://dx.doi.org/10.22110/jkums.v17i4.785

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