Comparison of homocysteine levels in amniotic fluid and simultaneously blood serum between severe preeclamptic women and control group

Shohreh Malek khosravi, Negin Rezavand, Maryam Zangeneh, Firoozeh Veisi, Neda Soleimani Abyaneh, Hamid Nomani, Mansour Rezaei, Mojgan Kohzadi


Background: previous studies have shown that preeclampsia is associated with elevated maternal serum homocysteine levels, but few studies have evaluated homocysteine levels in amniotic fluid in these patients. The purpose of this study was to compare amniotic fluid homocysteine levels in preeclamptic women, with those of normal pregnant women.

Methods: A cross sectional study was done on 46 pregnant women with severe preeclampsia and 46 normal pregnant women. Homocysteine levels in amniotic fluid and maternal serum were compared in two groups. Data were analyzed using t test and leven's test.

Results: The mean serum homocysteine levels in the third trimester of pregnancy in the preeclamptic women was 3.965 +/- 1.8 and in normal group was 3.05 +/- 1.87 which was statistically significant (P.v=0.019). The mean amniotic fluid homocysteine levels in the third trimester of pregnancy in mothers with severe preeclampsia was 11.11 +/- 7.41 and in the control group was 8.15 +/- 5.71, in which the difference was statistically significant (P.v=0.035). The average of differences between serum and amniotic fluid homocysteine levels in preeclamptic group was 7.14 +/- 6.01 and the average of differences in normal group was 5.1 +/- 4.34, there were no statistically significant differences between two groups (P.v=0.065)

Conclusion: In the patients with severe preeclampsia, homocysteine levels in serum and amniotic fluid were higher than those of normal pregnant women. Higher homocysteine levels in amniotic fluid of preeclamptic women could indicate the role of the fetus in producing of the preeclampsia.


Lavee M, Goldman Sh, Spiegel ED, Shalev E. Matrix metalloproteinase-2 is elevated in midtrimester amniotic fluid prior to the development of preeclampsia. Reprod Boil Endocrinol. 2009;7:85.

Wang Y, Walli AK, Schulze A, Blessing F, Fraunberger P, Thaler C, et al. Heparin-mediated extracorporeal low density lipoprotein precipitation as a possible therapeutic approach in preeclampsia. Transfus Apher Sci. 2006;35(2):103-10.

Sibai B, Dekker G, Kupferminc M. Preeclampsia. Lancet. 2005;365(9461):785-99.

Malek-khosravi S, Kaboudi M, Kaboudi B, Atefi G. Plasma homocysteine concentrations and insulin resistance in preeclampsia. Hypertension in Pregnancy. 2009;28:1-10.

Tug N, Celik H, Cikim G, Ozcelik O, Ayar A. The correlation between plasma homocysteine and malondialdehyde levels in preeclampsia. Neuroendocrinol Lett. 2003;24(6):445-8.

Aubard Y, Darodes N, Cantaloube M. Hyperhomocysteinemia and pregnancy-review of our present understanding and therapeutic implications. Eur J Obstet Gynecol Reprod Biol. 2000;93(2):157-65.

Sadia AM, Zamir A, Adina S, Qurrat-ul-Ain, Koukab S. Elevated circulating homocysteine level in patients with mild preeclampsia. Department of biochemistry services institute of medical sciences.

Powers RW, Evans RW, Majors AK, Ojimba JI, Ness RB, Crombleholme WR, et al. Plasma homocysteine concentration is increased in preeclampsia and associated with evidence of endothelial activation. Am J Obstet Gynecol. 1998;179(6 Pt 1):1605-11.

Hague WM. Homocystein and pregnancy. Best Pract Res Clin Obstet Gynaecol. 2003;17(3):459-69.

Ingec M, Borekci B, Kadanali S. Elevated plasma homocysteine concentrations in severe preeclampsia and eclampsia. Tohoku J. 2005;206:225-31.

Kajdy A, Niemiec T. Homocysteine metabolism disorders as potential predictor of preeclampsia. Ginekol Pol. 2008;79(11):775-9.

Calle M. de la, Usandizaga R, Sancha M, Magdaleno F, Herranz A, Cabrillo E. Homocysteine, folic acid and B-group vitamins in obstetrics and gynecology. Eur J Obstet Gynecol Reprod Biol. 2003;107(2):125-34.

Abramovich DR, Garden A, Jandial L, Page KR. Fetal swallowing and voiding in relation to hydramnios. Obstet Gynecol. 1979;54(1):15-20.

Walker MC, Smith GN, Perkins SL, Keely EJ, Garner PR. Changes in homocysteine levels during normal pregnancy. Am J Obstet Gynecol. 1999;180(3 Pt 1):660-4.

Hietala R, Turpeinen U, Laatikainen T. Serum homocysteine at 16 weeks and subsequent preeclampsia. Obstet Gynecol. 2001;97(4):527-9.

Katharine D. Wenstrom, Mda, Am J obstet Gynecol 2001, 184(5): 812-7.



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